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HLA Laboratory

The HLA laboratory performs testing of leukocyte antibodies (lymphocytotoxicity test, granulo-aglutination test, granuloimmuno-fluorescent test and examination of platelet antibodies (platelet immune-fluorescence test and ELISA test).

 Alloimmune antibodies emerge primarily as a result of repeated blood transfusions, after organ transplantations (of kidneys), in pregnancy and they are a frequently the cause of post transfusion reactions. For immunized patients it is recommended to receive leukodepleted blood transfusion products. For patients on the kidney transplantation waiting lists we perform examinations of HLA antibodies by a lymphocytotoxicity test four times a year (these are the so-called PRA panel of reactive antibodies). Auto-antibodies are irrelevant for transfusion and transplantation; they are often the cause of neutropenia and thrombocytopenia.

The typing of the HLA first class antigens (ie, HLA-A,-B and Cw) by serology method is performed in patients enrolled in the transplantation program, in leukemic patients and their family members.
To assist the Transplantation Center in Ostrava we perform the typing of cadaverous donors and a pre-transplantation examination, the so-called cross-match, which is a cross-test designed to determine the compatibility of the serum of the recipient with the donor lymphocytes. The positivity of this test is a contraindication for transplantation. The cross-match is performed before the selection of a suitable recipient on the frozen sera of all candidates for kidney transplantation, and again on the fresh serum of the selected patient immediately before transplantation (the current cross-match).

The donor lymphocytes are frozen so that we can repeat the cross-match when suspecting the humoral rejection of an organ.
The HLA laboratory is one of the six HLA laboratories in the Czech Republic that ensures and provides entry assessment into the Bone Marrow Donor Registry (ČNRDD) in Pilsen. This register, which is the largest of all the post-communist countries, has already over 30 000 registered donors.
The testing of the HLA first class antigens associated with diseases, the best known example is the HLA-B27 in Bekhterev’s disease (ankylosing spondylitis). If more first-class antigens are demanded, we investigate the whole typing of HLA-AB.